Hydrogen Peroxide Response in Leaves of Poplar (Populus simonii × Populus nigra) Revealed from Physiological and Proteomic Analyses.
Identifieur interne : 001354 ( Main/Exploration ); précédent : 001353; suivant : 001355Hydrogen Peroxide Response in Leaves of Poplar (Populus simonii × Populus nigra) Revealed from Physiological and Proteomic Analyses.
Auteurs : Juanjuan Yu [République populaire de Chine] ; Xin Jin [République populaire de Chine] ; Xiaomei Sun [République populaire de Chine] ; Tianxiang Gao [République populaire de Chine] ; Xiaomei Chen [République populaire de Chine] ; Yimin She [République populaire de Chine] ; Tingbo Jiang [République populaire de Chine] ; Sixue Chen [République populaire de Chine, États-Unis] ; Shaojun Dai [République populaire de Chine]Source :
- International journal of molecular sciences [ 1422-0067 ] ; 2017.
Descripteurs français
- KwdFr :
- Feuilles de plante (physiologie), Métabolisme glucidique (MeSH), Nucleoside diphosphate kinase (métabolisme), Peroxyde d'hydrogène (métabolisme), Photosynthèse (MeSH), Populus (physiologie), Protéines 14-3-3 (métabolisme), Protéines végétales (métabolisme), Protéomique (MeSH), Stress physiologique (MeSH), Transduction du signal (MeSH).
- MESH :
English descriptors
- KwdEn :
- 14-3-3 Proteins (metabolism), Carbohydrate Metabolism (MeSH), Hydrogen Peroxide (metabolism), Nucleoside-Diphosphate Kinase (metabolism), Photosynthesis (MeSH), Plant Leaves (physiology), Plant Proteins (metabolism), Populus (physiology), Proteomics (MeSH), Signal Transduction (MeSH), Stress, Physiological (MeSH).
- MESH :
- chemical , metabolism : 14-3-3 Proteins, Hydrogen Peroxide, Nucleoside-Diphosphate Kinase, Plant Proteins.
- physiology : Plant Leaves, Populus.
- Carbohydrate Metabolism, Photosynthesis, Proteomics, Signal Transduction, Stress, Physiological.
Abstract
Hydrogen peroxide (H₂O₂) is one of the most abundant reactive oxygen species (ROS), which plays dual roles as a toxic byproduct of cell metabolism and a regulatory signal molecule in plant development and stress response. Populus simonii × Populus nigra is an important cultivated forest species with resistance to cold, drought, insect and disease, and also a key model plant for forest genetic engineering. In this study, H₂O₂ response in P. simonii × P. nigra leaves was investigated using physiological and proteomics approaches. The seedlings of 50-day-old P. simonii × P. nigra under H₂O₂ stress exhibited stressful phenotypes, such as increase of in vivo H₂O₂ content, decrease of photosynthetic rate, elevated osmolytes, antioxidant accumulation, as well as increased activities of several ROS scavenging enzymes. Besides, 81 H₂O₂-responsive proteins were identified in the poplar leaves. The diverse abundant patterns of these proteins highlight the H₂O₂-responsive pathways in leaves, including 14-3-3 protein and nucleoside diphosphate kinase (NDPK)-mediated signaling, modulation of thylakoid membrane structure, enhancement of various ROS scavenging pathways, decrease of photosynthesis, dynamics of proteins conformation, and changes in carbohydrate and other metabolisms. This study provides valuable information for understanding H₂O₂-responsive mechanisms in leaves of P. simonii × P. nigra.
DOI: 10.3390/ijms18102085
PubMed: 28974034
PubMed Central: PMC5666767
Affiliations:
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Juanjuan" uniqKey="Yu J" first="Juanjuan" last="Yu">Juanjuan Yu</name><affiliation wicri:level="1"><nlm:affiliation>Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, Harbin 150040, China. yujuan8186@163.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, Harbin 150040</wicri:regionArea><wicri:noRegion>Harbin 150040</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Development Centre of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China. yujuan8186@163.com.</nlm:affiliation><country xml:lang="fr">République populaire de 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200234</wicri:noRegion></affiliation></author><author><name sortKey="Gao, Tianxiang" sort="Gao, Tianxiang" uniqKey="Gao T" first="Tianxiang" last="Gao">Tianxiang Gao</name><affiliation wicri:level="1"><nlm:affiliation>Development Centre of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China. thalia1@163.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Development Centre of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234</wicri:regionArea><wicri:noRegion>Shanghai 200234</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Xiaomei" sort="Chen, Xiaomei" uniqKey="Chen X" first="Xiaomei" last="Chen">Xiaomei Chen</name><affiliation wicri:level="1"><nlm:affiliation>Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, Shanghai 201602, China. chenxm@sibs.ac.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, Shanghai 201602</wicri:regionArea><wicri:noRegion>Shanghai 201602</wicri:noRegion></affiliation></author><author><name sortKey="She, Yimin" sort="She, Yimin" uniqKey="She Y" first="Yimin" last="She">Yimin She</name><affiliation wicri:level="1"><nlm:affiliation>Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, Shanghai 201602, China. ymshe@sibs.ac.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, Shanghai 201602</wicri:regionArea><wicri:noRegion>Shanghai 201602</wicri:noRegion></affiliation></author><author><name sortKey="Jiang, Tingbo" sort="Jiang, Tingbo" uniqKey="Jiang T" first="Tingbo" last="Jiang">Tingbo Jiang</name><affiliation wicri:level="1"><nlm:affiliation>Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, Harbin 150040, China. tbjiang@yahoo.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, Harbin 150040</wicri:regionArea><wicri:noRegion>Harbin 150040</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Sixue" sort="Chen, Sixue" uniqKey="Chen S" first="Sixue" last="Chen">Sixue Chen</name><affiliation wicri:level="1"><nlm:affiliation>Development Centre of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China. schen@ufl.edu.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Development Centre of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234</wicri:regionArea><wicri:noRegion>Shanghai 200234</wicri:noRegion></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Biology, Genetics Institute, Plant Molecular and Cellular Program, Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL 32610, USA. schen@ufl.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biology, Genetics Institute, Plant Molecular and Cellular Program, Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL 32610</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Dai, Shaojun" sort="Dai, Shaojun" uniqKey="Dai S" first="Shaojun" last="Dai">Shaojun Dai</name><affiliation wicri:level="1"><nlm:affiliation>Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, Harbin 150040, China. daishaojun@hotmail.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, Harbin 150040</wicri:regionArea><wicri:noRegion>Harbin 150040</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Development Centre of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China. daishaojun@hotmail.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Development Centre of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234</wicri:regionArea><wicri:noRegion>Shanghai 200234</wicri:noRegion></affiliation></author></analytic><series><title level="j">International journal of molecular sciences</title><idno type="eISSN">1422-0067</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>14-3-3 Proteins (metabolism)</term><term>Carbohydrate Metabolism (MeSH)</term><term>Hydrogen Peroxide (metabolism)</term><term>Nucleoside-Diphosphate Kinase (metabolism)</term><term>Photosynthesis (MeSH)</term><term>Plant Leaves (physiology)</term><term>Plant Proteins (metabolism)</term><term>Populus (physiology)</term><term>Proteomics (MeSH)</term><term>Signal Transduction (MeSH)</term><term>Stress, Physiological (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Feuilles de plante (physiologie)</term><term>Métabolisme glucidique (MeSH)</term><term>Nucleoside diphosphate kinase (métabolisme)</term><term>Peroxyde d'hydrogène (métabolisme)</term><term>Photosynthèse (MeSH)</term><term>Populus (physiologie)</term><term>Protéines 14-3-3 (métabolisme)</term><term>Protéines végétales (métabolisme)</term><term>Protéomique (MeSH)</term><term>Stress physiologique (MeSH)</term><term>Transduction du signal (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>14-3-3 Proteins</term><term>Hydrogen Peroxide</term><term>Nucleoside-Diphosphate Kinase</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Nucleoside diphosphate kinase</term><term>Peroxyde d'hydrogène</term><term>Protéines 14-3-3</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Feuilles de plante</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Carbohydrate Metabolism</term><term>Photosynthesis</term><term>Proteomics</term><term>Signal Transduction</term><term>Stress, Physiological</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Métabolisme glucidique</term><term>Photosynthèse</term><term>Protéomique</term><term>Stress physiologique</term><term>Transduction du signal</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Hydrogen peroxide (H₂O₂) is one of the most abundant reactive oxygen species (ROS), which plays dual roles as a toxic byproduct of cell metabolism and a regulatory signal molecule in plant development and stress response. <i>Populus simonii</i> × <i>Populus nigra</i> is an important cultivated forest species with resistance to cold, drought, insect and disease, and also a key model plant for forest genetic engineering. In this study, H₂O₂ response in <i>P. simonii × P. nigra</i> leaves was investigated using physiological and proteomics approaches. The seedlings of 50-day-old <i>P. simonii × P. nigra</i> under H₂O₂ stress exhibited stressful phenotypes, such as increase of in vivo H₂O₂ content, decrease of photosynthetic rate, elevated osmolytes, antioxidant accumulation, as well as increased activities of several ROS scavenging enzymes. Besides, 81 H₂O₂-responsive proteins were identified in the poplar leaves. The diverse abundant patterns of these proteins highlight the H₂O₂-responsive pathways in leaves, including 14-3-3 protein and nucleoside diphosphate kinase (NDPK)-mediated signaling, modulation of thylakoid membrane structure, enhancement of various ROS scavenging pathways, decrease of photosynthesis, dynamics of proteins conformation, and changes in carbohydrate and other metabolisms. This study provides valuable information for understanding H₂O₂-responsive mechanisms in leaves of <i>P. simonii</i> × <i>P. nigra</i>.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28974034</PMID><DateCompleted><Year>2018</Year><Month>05</Month><Day>29</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1422-0067</ISSN><JournalIssue CitedMedium="Internet"><Volume>18</Volume><Issue>10</Issue><PubDate><Year>2017</Year><Month>Oct</Month><Day>02</Day></PubDate></JournalIssue><Title>International journal of molecular sciences</Title><ISOAbbreviation>Int J Mol Sci</ISOAbbreviation></Journal><ArticleTitle>Hydrogen Peroxide Response in Leaves of Poplar (Populus simonii × Populus nigra) Revealed from Physiological and Proteomic Analyses.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E2085</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/ijms18102085</ELocationID><Abstract><AbstractText>Hydrogen peroxide (H₂O₂) is one of the most abundant reactive oxygen species (ROS), which plays dual roles as a toxic byproduct of cell metabolism and a regulatory signal molecule in plant development and stress response. <i>Populus simonii</i> × <i>Populus nigra</i> is an important cultivated forest species with resistance to cold, drought, insect and disease, and also a key model plant for forest genetic engineering. In this study, H₂O₂ response in <i>P. simonii × P. nigra</i> leaves was investigated using physiological and proteomics approaches. The seedlings of 50-day-old <i>P. simonii × P. nigra</i> under H₂O₂ stress exhibited stressful phenotypes, such as increase of in vivo H₂O₂ content, decrease of photosynthetic rate, elevated osmolytes, antioxidant accumulation, as well as increased activities of several ROS scavenging enzymes. Besides, 81 H₂O₂-responsive proteins were identified in the poplar leaves. The diverse abundant patterns of these proteins highlight the H₂O₂-responsive pathways in leaves, including 14-3-3 protein and nucleoside diphosphate kinase (NDPK)-mediated signaling, modulation of thylakoid membrane structure, enhancement of various ROS scavenging pathways, decrease of photosynthesis, dynamics of proteins conformation, and changes in carbohydrate and other metabolisms. This study provides valuable information for understanding H₂O₂-responsive mechanisms in leaves of <i>P. simonii</i> × <i>P. nigra</i>.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Juanjuan</ForeName><Initials>J</Initials><Identifier Source="ORCID">0000-0002-7209-0645</Identifier><AffiliationInfo><Affiliation>Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, Harbin 150040, China. yujuan8186@163.com.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Development Centre of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China. yujuan8186@163.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jin</LastName><ForeName>Xin</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, Harbin 150040, China. JinXin721612@163.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Xiaomei</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Development Centre of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China. meimeixpz@163.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gao</LastName><ForeName>Tianxiang</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Development Centre of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China. thalia1@163.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Xiaomei</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, Shanghai 201602, China. chenxm@sibs.ac.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>She</LastName><ForeName>Yimin</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, Shanghai 201602, China. ymshe@sibs.ac.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Tingbo</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, Harbin 150040, China. tbjiang@yahoo.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Sixue</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Development Centre of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China. schen@ufl.edu.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Biology, Genetics Institute, Plant Molecular and Cellular Program, Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL 32610, USA. schen@ufl.edu.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dai</LastName><ForeName>Shaojun</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, Harbin 150040, China. daishaojun@hotmail.com.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Development Centre of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China. daishaojun@hotmail.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>10</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Int J Mol Sci</MedlineTA><NlmUniqueID>101092791</NlmUniqueID><ISSNLinking>1422-0067</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D048948">14-3-3 Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>BBX060AN9V</RegistryNumber><NameOfSubstance UI="D006861">Hydrogen Peroxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.4.6</RegistryNumber><NameOfSubstance UI="D009701">Nucleoside-Diphosphate Kinase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D048948" MajorTopicYN="N">14-3-3 Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D050260" MajorTopicYN="N">Carbohydrate Metabolism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006861" MajorTopicYN="N">Hydrogen Peroxide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009701" MajorTopicYN="N">Nucleoside-Diphosphate Kinase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010788" MajorTopicYN="N">Photosynthesis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015398" MajorTopicYN="N">Signal Transduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="N">Stress, Physiological</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">H2O2 stress</Keyword><Keyword MajorTopicYN="N">Populus simonii × Populus nigra</Keyword><Keyword MajorTopicYN="N">leaves</Keyword><Keyword MajorTopicYN="N">proteomics</Keyword></KeywordList><CoiStatement>The authors declare no conflict of interest.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate 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